Journal: bioRxiv

Article Title: Direct interaction of ribosomes with postsynaptic proteins gives rise to a privileged local synaptic translatome

doi: 10.64898/2026.02.27.708433

Figure Lengend Snippet: ( A ) Schematic of XL-MS workflow. ( B ) XL-based interactome of synaptic ribosomes. Synaptic annotation is based on SynGO (dataset version 20231201). ( C ) Protein domain-level crosslink map between RPL35 and CaMKIIα. Domain classification based on . ( D ) Structural model of CaMKIIα binding to the ribosome. Most CaMKIIα–RPL35 crosslinks satisfy distance constraints (green), with remaining links accommodated by CaMKIIα linker flexibility (yellow). ( E ) Detection of newly-synthesized proteins in dendrites and synapses from control (untreated) neurons or neurons treated with 10 μM KN-93 or myr-AIP for 30 min. Nascent proteins were labeled with puromycin (5 min) in the absence or presence of the protein synthesis inhibitor anisomycin (see Methods). Scale bar, 5 µm. ( F ) Quantification of puromycin signal in postsynaptic regions of the apical dendritic arbor from control and treated neurons. Error bars show mean ± SEM. **p<0.01; ****p<0.0001, Brown-Forsythe and Welch’s ANOVA, Dunnett’s multiple comparisons test; n=58-64 neurons from 3 independent cultures.

Article Snippet: DIV 18-19 hippocampal neurons were treated with either 10 μM KN-93 (Tocris, #5215) or 10 μM myr-AIP (Tocris, #5959) for 30 min at 37°C, or left untreated.

Techniques: Structural Proteomics, Binding Assay, Synthesized, Control, Labeling

Journal: bioRxiv

Article Title: Bitter taste receptor T2R14 and autophagy flux in gingival epithelial cells

doi: 10.1101/2024.01.17.576143

Figure Lengend Snippet: WT, T2R14KO, ATG7 KD and T2R14KO-ATG7KD cells (approx. 30,000/96 wells) were loaded with Fluo-4NW calcium dye and intracel cellular calcium dye and intracell calcium response was measured after Baf A1 and rapa treatment. Data shown are mean ± SEM from three independent experiments done in triplicate. B. Effect of S. aureus auto inducer peptide (AIP) and S. mutans competence stimulating peptide (CSP-1) on calcium mobilization in GEC cells . Intracellular calcium release measurement after application of autoinducer peptides (AIP from S. aureus and CSP-1 from S. mutans 50μM each) in GEC cells. Results are from three independent experiments done in triplicate. C . Combined effect of Baf A1 and CSP-1 on calcium signalling in GEC. WT, T2R14KO, ATG7 KD and T2R14KO-ATG7KD cells (approx. 30,000/96 wells) were loaded with Fluo-4NW calcium dye and intracellular calcium response was measured after CSP-1 and CSP-1 plus Baf A1 treatment. Data shown are mean ± SEM from three independent experiments done in triplicate.

Article Snippet: Synthetic S. mutans competence stimulating peptide (CSP-1) and S. aureus auto inducer peptide (AIP) of 98% purity was purchased from GenScript (Piscataway, NJ, USA).

Techniques: